Bone Abstracts

Osteoclasts are multinucleated bone-resorbing cells and can be cultured from different monocytic precursors. It is unknown whether osteoclasts derived from different precursors are phenotypically different. One of the aspects not known is the life span of the different osteoclasts and the effect of IL-1β hereupon. Here, we studied this using time-lapse microscopy. Bone marrow cells were isolated from 6-week-old male mice. Early blasts (CD31hiLy-6C-),...

Background: Osteoclasts (OCs) are bone-degrading cells that differentiate from the monocyte/macrophage lineage. In human, three monocyte subsets have been identified: classical, intermediate and non-classical monocytes. We have previously demonstrated that comparable numbers of OCs can be generated from these subsets on plastic, but that the number of OCs significantly differs when the monocytes are cultured on bone. It is plausible that the observed differences are associated...